Chargaff found out that t<span>he ratios of thymine and adenine were equal and the ratios of guanine and cytosine were equal. He then created the Chargaff's rule states that double stranded DNA has a 1:1 ratio between pyrimidine and purine bases. With this, </span><span>amount of guanine bases is equal to cytosine bases while the amount of adenine bases is equal to thymine bases.</span>