Answer:
Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution, such as STED, GSD, RESOLFT and SSIM.
Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behavior, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time, such as Super-resolution optical fluctuation imaging (SOFI) and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.
Explanation:
https://www.creative-biostructure.com/Super-resolution-Microscopy-Service-590.htm
Why are they being abused?
Does a animal that is going to be used for human consumption deserve to be treated cruelly?
Does the USDA regulate pig farms and treatment of the pigs?
Are there sanctions for cruelty?
How are the pigs slaughtered, humanly or not?
Protist and fungi both have multi and unicellular organisms
