You should mix
4µl of DNA sample and complete it with
96µl (100-4) of water.
You can figure this out by using
C1.V1 = C2.V2C1 = 250 <span>ng/μl (Sample Concentration)
</span>V1 = ?
(the necessary volume to take from the DNA sample)C2 = 10 <span>ng/μl (PCR concentration)
</span>V2 = 100 µl (total volume)
V1 =

=

=
4µl
For the water: 100µl (total volume) = 4µl + Water
Water volume = 96µl
Because: Some of the work done by the machine is used to overcome the friction created by the use of the machine. ... Work output can never be greater than work input. Machines allow force to be applied over a greater distance, which means that less force will be needed for the same amount of work.
Hope this helps!
The stomach can sometimes expires problem when digesting hard foods such as cheese and may result in things such as heart burn. an antacid can counter the ragging stomach acids.