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Paraphin [41]
3 years ago
13

Outline an experimental approach to determining the average chain growth rate for transcription in vivo. Chain growth rate is th

e number of nucleotides polymerized per minute per RNA chain. Fill in the blanks.
Label a growing bacterial or cell culture for a brief interval with a mixture of 14C-labeled nucleosides, which would be converted to nucleotides and incorporated into _____. Isolate total ______and subject it to hydrolysis with mild _____. Every _____-terminal nucleotide will be released as a nucleoside. Separate nucleosides from nucleotides, by ______. Total picomoles of nucleotide in the hydrolysate divided by total picomoles of nucleoside gives the number of nucleotides incorporated per _______chain-which, when divided by the labeling interval (minutes) gives the growth rate of individual chains (nucleotides incorporated per ______ chain per minute). An interesting variant of the experiment would be to carry it out with individual radiolabeled nucleosides and ask whether the chain ______is the same for each nucleotide.
Biology
1 answer:
baherus [9]3 years ago
6 0

Answer:

DNA

Protein

temperature

nerve

esterification of inorganic phosphate to C5 on aldose.

nucleic acid polymer

growth rate

Explanation:

Nucleosides have nitrogen base surrounded by 5 carbon carbohydrates which is called as ribose molecule. Nucleotides is simply a nucleoside with one or more phosphate group. There are 4 different types of nucleosides which are, adenosine,  guanosine cytidine, and uridine. Nucleotides are formed from nucleosides by esterification of inorganic phosphate on aldose.

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luda_lava [24]

The movement of carbon through the abiotic and biotic parts of the carbon cycle is the following:

In the carbon cycle, carbon is moved between the four reservoirs where it is in different states:

  • Atmosphere, carbon is in the form of carbon dioxide (CO2) by joining with oxygen in the form of gas.
  • Terrestrial biosphere, carbon is found in the elements that make up terrestrial and coastal ecosystems, in non-living organic matter, and in the soil.
  • Oceans, carbon is part of the hydrosphere, it is found in dissolved organic carbon, in marine organisms and in non-living matter.

The water vapor produced and released into the atmosphere also promotes gas exchange between the atmosphere and the ocean, allowing carbon to dissolve in the water, where it is fixed by photosynthetic plankton.

  • Sediments: carbon is part of the geosphere, the excess carbon in decomposing organic matter, which is not captured and processed by decomposing life forms, it will pile up and sediment at the bottom of the oceans or in the various layers of the earth's crust, where it forms fossils, hydrocarbon deposits or reactive sediments.

During photosynthesis, green plants take CO2 from the abiotic environment and incorporate the carbon into the carbohydrates they synthesize.

Part of these carbohydrates are metabolized by the same producers in their respiration, returning carbon to the surrounding environment in the form of CO2.

That is, the return of CO2 to the atmosphere occurs when living beings oxidize food while breathing, producing CO2.

The final products of combustion are CO2 and water vapor.

Therefore, we can conclude that the movement of carbon between the abiotic environment and biotic factors (living organisms) defines the carbon cycle.

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7 0
3 years ago
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Sexual reproduction is the process in which new organisms are created, by combining the genetic information from two individuals of different sexes.

Explanation:

The genetic information is carried on chromosomes within the nucleus of specialized sex cells called gametes.

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