Outline an experimental approach to determining the average chain growth rate for transcription in vivo. Chain growth rate is th
e number of nucleotides polymerized per minute per RNA chain. Fill in the blanks. Label a growing bacterial or cell culture for a brief interval with a mixture of 14C-labeled nucleosides, which would be converted to nucleotides and incorporated into _____. Isolate total ______and subject it to hydrolysis with mild _____. Every _____-terminal nucleotide will be released as a nucleoside. Separate nucleosides from nucleotides, by ______. Total picomoles of nucleotide in the hydrolysate divided by total picomoles of nucleoside gives the number of nucleotides incorporated per _______chain-which, when divided by the labeling interval (minutes) gives the growth rate of individual chains (nucleotides incorporated per ______ chain per minute). An interesting variant of the experiment would be to carry it out with individual radiolabeled nucleosides and ask whether the chain ______is the same for each nucleotide.
esterification of inorganic phosphate to C5 on aldose.
nucleic acid polymer
growth rate
Explanation:
Nucleosides have nitrogen base surrounded by 5 carbon carbohydrates which is called as ribose molecule. Nucleotides is simply a nucleoside with one or more phosphate group. There are 4 different types of nucleosides which are, adenosine, guanosine cytidine, and uridine. Nucleotides are formed from nucleosides by esterification of inorganic phosphate on aldose.
