Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Animal cells communicate via their extracellular matrices and are connected to each other via tight junctions, desmosomes, and gap junctions
So we know that to transport materials in or out of the cell, we need to have access to both the inside and outside of the cell. This would require that the protein be a transmembrane protein that reaches both the inside and the outside of the cell.
So in this case, let's look at pore proteins. These are proteins that cross a membrane and act as a pore for the materials that need to cross the membrane.
One example of a pore protein is an aquaporin. These proteins aid in the transport of water into or out of a cell.
Therefore, the answer to your question is: A) Pore proteins.
It is kind of a strange question but by the process of elimination, I would go with petrification.
Its called petrified wood.
hope I Helped
Answer:
The correct answer is - false.
Explanation:
Penicillin-binding protein 2a or PBPs are protein produced by bacteria that binds with the β-lactam antibiotics such as penicillin. They play role in the cell wall synthesis of bacteria by producing peptidoglycan by catalyzing the reaction.
β-Lactam antibiotics bind with the PBPs and cause the disruption to peptidoglycan cross-linking in the biosynthesis of the cell wall and ultimately lysis of bacteria takes place and cell death.
