Answer:
The two important conditions that must be met before gram staining are-
1. The age of the culture should not exceed 24 hours.
2. Over heating, decolonization should be avoided.
Explanation:
Gram staining is a technique developed to distinguish the bacteria on the basis of the composition of the cell wall around the bacteria.
The gram stain is a sensitive technique so must be performed carefully. The precautions should be taken to avoid any error while gram staining that is-
1. Age of the culture- The culture should be freshly prepared just 18-24 hours before the experiment as above 24 hours bacteria lose their ability to retain the color so one would not be able to differentiate between the cultures.
2. While performing the experiment one should avoid overheating and decolorization with ethanol as both the factors lead to the loss of crystal violet stain from the cell and they take pinkish color after safranin staying.
What followings ? You have no options for us to pick from.
Answer:
the physical distance between the two genes.
Explanation:
The primary products of the light independent reactions (after one turn of the cycle) are:
a) two G3P molecules
b) three ADP
c) two NADP+
However ADP and NADP+ are not really "products". They are regenerated and later used again in the Light-dependent reactions. Each G3P molecule is composed of three carbons.
For the Calvin cycle (Light independent cycle) to continue, 5 out of the 6 carbons provided by the two G3P molecules are used to regenerate ribulose 1, 5 phosphate. Therefore there remains only one carbon for the next turn of the cycle.
One molecule of glucose requires 6 turns of the cycle. Any extra G3P is used to make starch, sucrose and cellulose.
