1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
xeze [42]
4 years ago
11

For each scenario determine which type of column chromatography would be the best choice to separate these proteins. For each wh

ich proteins would elute off of the column first? Please explain your answers. a) Protein A: LLLGGAAPPVLHKWMMAPG or Protein B: HHKGLARTIRKHLLIMGASHK b) Protein A: LLLGGAAPPVLHK WMMAPG or Protein B: VVLTCNMI
Biology
1 answer:
Korvikt [17]4 years ago
7 0

Answer:

The correct answers are:

a) "cation exchange chromatography, Protein A will elute first"

b) "size exclusion chromatography, Protein A will elute first".

Explanation:

Liquid chromatography is a common method for separation of proteins, which is based on the differences of affinity that the proteins have to a column. For the separation of the two first proteins the best choice will be to use a cation exchange chromatography, a method that uses a column that is negatively charged. Protein A will be the first to elute because Protein B has more affinity to the column because it is highly positive charged at neutral pH due to its high content of histidine and lysine residues. Regarding the separation of the second group of proteins, the best choice will be to use a size exclusion chromatography. In this technique the proteins are separated based on its molecular size, and the first one to elute will be the one that has a bigger size, which is Protein A.

You might be interested in
Ecologists use survivorship curves to visualize how the number of individuals in a population drops off with time. By plotting t
Flura [38]

Answer:

The wrong statement is C. <em>Individuals with Type Il survivorship exhibit high survivorship throughout </em>

their life cycle.

Explanation:

Different species have survival curves differently shaped. In general terms, there are three different survival curves.

  • Type I. Organisms do not tend to die when they are young or middle-aged, but they do when they are old. These species, in general, have a few descendants and parents provide much parental care to ensure their progeny survival. Typical of K selected species.
  • Type II. Organisms that have more or less the same probabilities of dying in each age interval. These organisms can also have a few descendants and they can provide significant parental care.
  • Type III. Only a few individuals survive their first period of life or their firsts years. However, the lucky ones to reach a certain age generally have a long life. These organisms have a lot of descendants at the same time, but they do not provide much parental care. Typical of r selected species.
6 0
4 years ago
Thick skin has a more extensive dermal layer than thin skin. thick skin has a more extensive dermal layer than thin skin.
eduard

Answer:

false

Explanation:

thin skin actually has more extensive dermal layer - this make thin skin easier to stitch while thick skin is found on fingertips & soles of the feet

7 0
4 years ago
Which of the following is added to ocean water as a result of respiration?
gizmo_the_mogwai [7]

Answer:

Its CO2

Explanation:

All marine organisms use oxygen for respiration, which releases energy from carbohydrates and has carbon dioxide and water as byproducts

6 0
3 years ago
Messenger RNA carries a(n) ___________ of the DNA’s instructions out of the nucleus to the ___________.
AleksandrR [38]

Answer: The correct option for the fill in the blank is  B) Transcript, ribosome.

mRNA is formed by a template of DNA in the nucleus of eukaryotic cells. The genetic information from DNA is transferred to mRNA in process called Transcription. mRNA is transported outside nucleus and helps in protein synthesis at the site called ribosomes.

mRNA thus carries a transcript of DNA instruction out of the nucleus. It helps in the formation of synthesis at ribososme ( present in the cytoplasm of the cell).

Thus, option B) is the right answer.

8 0
4 years ago
___ energy is energy that has not yet been used
xxMikexx [17]

Answer:

Potential energy is energy that has not been used

4 0
3 years ago
Other questions:
  • All animals and plants have adaptations to help them survive, but some of these adaptations can be quite bizarre. Find out and e
    7·1 answer
  • Which molecule contains the genetic code?
    5·1 answer
  • WILL GIVE YOU BRAINIEST!!
    5·1 answer
  • What kind of research method is are these?
    15·1 answer
  • How are photosynthesis and cellular respiration related?
    9·2 answers
  • How do plankton differ from nekton? plankton are floaters. plankton are strong swimmers. plankton live on the ocean bottom. plan
    10·2 answers
  • The instructions for making proteins are coded in the what?
    15·2 answers
  • Describe the trophic structure of energy in an ecosystem
    15·1 answer
  • Name the disease caused by a deficiency in iron.
    9·2 answers
  • A fold is a ------------ in rock, and a fault is a -------------- in rock.
    6·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!