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Alisiya [41]
3 years ago
14

1. After examining the DNA of different organisms, which of the following did Erwin Chargaff conclude about the four bases?

Biology
1 answer:
siniylev [52]3 years ago
4 0

Answer: a). A = T and C = G.

Explanation: Erwin Chargaff's rule states that in a double-stranded DNA molecule, the number of adenine is equal to the number of thymine and the number of cytosine is equal to the number of guanine. This means that there is a ratio purine to pyrimidine in a double-stranded DNA molecule is 1:1.

There are four bases found in the DNA: adenine, thymine, guanine and cytosine and according to the Watson and Crick base pairing rule, adenine pairs with thymine while guanine pairs with cytosine. Adenine and guanine are the purine bases while thymine and cytosine are pyrimidine bases.

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Many mutations have been identified in the lac operon. A particular mutation in the CRP gene make the CRP protein bind to the Pl
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a. + glucose, + lactose  = On

b. - glucose, - lactose  = Off

c. + glucose, - lactose  = Off

d. - glucose, + lactose = On

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Lac operon has both types of control, repressible and inducible.

Whenever glucose level is low in the cell, an enzyme known as adenylyl cyclase raises the level of cAMP which forms a dimer with CRP protein and they both act as activator of lac operon and cause expression.

Apart from this, when lactose is present in the cell, β-galactosidase enzyme metabolizes lactose to form allolactose which causes allosteric repulsion in the lac repressor and causes its removal from the operator. As soon as repressor is removed lac operon gets activated.

In wild type lac operons, the expression of lac operon occurs when glucose level is low in the cell and lactose is present but in this mutant presence or absence of glucose will not make a difference because CRP will bind Plac promoter independent of cAMP level i.e. activator CRP will work even in high glucose concentration. If lactose is present then lac operon will always express so in option 'a & d' lac operon will express but in option 'b & c' it will not express.

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