While the absorbance at 420 nm is related to the amount of o-nitrophenol produced, the absorbance at 600 nm is proportional to cell density, which aids in standardizing our estimations of enzyme activity.
<h3>Describe absorbance.</h3>
The amount of light absorbed by a solution is measured by its absorbance (A), often referred to as optical density (OD). The amount of light that may flow through a solution is called its transmittance.
<h3>How is the activity of beta-galactosidase determined?</h3>
The colorless ONPG substrate is changed by beta-Galactosidase into galactose and the chromophore o-nitrophenol, which results in a vivid yellow solution. The amount of substrate transformed at 420 nm can be calculated by measuring the solution's beta-galactosidase activity using a spectrophotometer or a microplate reader.
<h3>What is measured by the beta-galactosidase assay?</h3>
The -Gal Assay Kit gives users the tools they need to swiftly assess the amounts of active beta-galactosidase expressed in cells that have been transfected with plasmids encoding the lacZ gene.
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Answer:
Its B I think (I'm not 100% sure)
Explanation:
Only 10% of the energy is transferred from one level to other,
so,
10% of 1,000J = 100 J
Which means 1000-100 = 900J of energy is used upon eating the snake.
(I’m not 100% sure about this answer though, but I think it will give u a good idea about energy transfer)
No, you cannot determine the properties of a chemical compound by solely knowing the properties of the elements that make up the compound, because in a chemical compound, the properties are completely different and or independent of the chemical and or physical properties of the individual elements used to make up the compound.
Answer: Indeed thanks for the free points.
