Answer:
The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
Explanation:
- Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
- These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
- Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
- Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
- At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
- This can cause the protein to get denatured and hence non-functional.
- Hence, it is never advisable to preserve REs by freezing directly without glycerol.
- Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.
Answer:
The correct option is A
Explanation:
To determine if water temperature has an effect on weathering of sedimentary rocks, different number (perhaps three) of identical sedimentary rocks should be obtained and then individually placed in different water temperatures (with at least one of the "water volumes" used for each having a temperature close to the room temperature so as to be used as the control).
Thus, each of the identical sedimentary rock can be broken into three and placed individually; as in one part of the rock in 20 °C of water (control), another part of the rock in 40 °C and another in 60 °C of water.
This above procedure should be repeated for the remaining two other identical sedimentary rock to confirm if there is any effect.
IN THE LUMEN INSIDE OF THE ENDOPLASMIC RECTICULUM.
<u>Explanation:</u>
The endoplasmic recticulum is the continuous membrane system that forms that forms the more number of flattened within the cytoplasm of eukaryotic cells and performs the multiple process in the cell.
The immportant functions of endoplasmic recticulum is folding, synthesis, modification, ans transport of the protein. The lumen is the protein which is present in the endoplasmic recticulum.
The lumen of the endoplasmic recticulum is the area closed by the endoplasmic recticulum membrane, it is an extensive network of the membrane tubues, visicles, and flattened the cisternae found in the eukaryotic cells.
1. Moles Fe = 209.7 g/ 55.847 g/mol= 3.75
The ratio between Fe and CO is 2 : 3
Moles CO = 3.75 x 3 /2 = 5.63
<span>Mass CO = 5.63 mol x 28.01 g/mol= 157.7 g
2. </span><span />2Na + 2H2O --> 2NaOH + H2<span>atomic weight of sodium is 23 48.7/23 = 2.12 moles of sodium
2.12/2 x 24 = 25.44dm^3 hydrogen = 1.06 moles
so multiply it by Avogadro's number1.06 X 6.02x10^23 = 1.204x10^24 molecules of hydrogen
</span>
I think the answer is Global Development Engineering.
People in this career help sustainable development happen through the use of their inventions that are mainly energy efficienct.
They are focused on research, design, and the creation of alternative ways to promote good health and sanitation, housing, and renewable energy that will cater developing or underdeveloped countries.
GLE is one engineering team that made a solar project in Africa.
