C2H2 is your answer for double bind
Chinese hamster ovary cell production of recombinant tissue‐type plasminogen activator (t‐PA) was increased by amplification of cotransfected dihydrofolate reeducates cDNA using stepwise adaptation to increasing methotrexate (MTX) concentrations. The highest producing clones were isolated at 5 μM MTX and yielded 26,000 U/106 cells/day t‐PA (43 μg/106 cells/day). Above 25 μM MTX, cell specific t‐PA production rates became increasingly variable and the cDNA copynumbers decreased. No apparent correlation between the cell specific t‐PA production rate and the growth rate was observed upon sub cloning of the amplified cells. When MTX selection was removed, the t‐PA production rate decreased up to tenfold within 40 days; this was accompanied by an up to 60% drop in cDNA copynumber. Subclones isolated after 108 days of culture in the absence of MTX were, on average, sixfold more stable than their parental cells. In culture without MTX, the maximum stable t‐PA production rate obtained (over 250 days) was 7000 ± 750 U/106cells/day (∼12 μg/106 cells/day), approximately threefold lower than the maximum unstable levels of production reached under selective pressure. Taken together, these results define a wide range of the highest t‐PA expression rates obtained under MTX selection, for which stable expression without selection has not been reported
Answer:
limbic system, which is located in the temporal lobe.
Answer:
Option (b).
Explanation:
The new life form should show the new characteristics that has not been earlier shown by the other life forms. The newly discovered life form should also shows the characteristics that are shown by the living organism.
The putative life form should show different anatomical structure or different way to react towards a stimulus. The bilateral symmetry can not be considered as a new life form because may animals are bilaterally symmetrical and this is not a new characteristic.
Thus, the correct answer is option (b).
Answer:
The correct answer is The bridge between cystein which connects parts of a protein is stable but non covalent interaction.
Explanation:
Disulfide bond link the sulfhydryl group of two adjacent cystein residues in a protein structure.
Dulsulfine bond is stable but is not a non covalent interaction.Disulfide bond itself is a covalent interaction.