Classical Biotechnology methods include: Selective Breeding, hybridization, and induced mutations, and recombinant DNA.
I feel like nobody answers biology Nd it’s irri but like ion know it
Answer:
The Ti plasmid is used as a vector for the transfer of genes in the plants. They are known to cause the crown gall disease in the plants. They have important economic value.
The transformation of the plant require the removal of tumor inducing gene for the formation of the disarmed pathogen. The gene of interest is kept in between the right and left border of the transfer DNA. This DNA is then introduced in the Agrobacterium. Then, it is cultivated with the target cells like callus so that the DNA can intgrate in the cells. The transformed cells are grown in a particular plants.