Answer:
The best way to find out if the amoeba and paramecium are alive is to look at them under the microscope and see them moving. The E coli and Bacillus you can spread on nutrient agar in a Petri dish and see if they form colonies.
Explanation:
The most famous cancer cells are called HeLa cells. They're named HeLa cells after the young woman whose cervical cancer cells were biopsied to create this immortal line of cells: Henrietta Lacks. Her cells are still used to today in cancer research.
Side note: Her cells were used WITHOUT her consent and without any type of compensation for her contribution. Her case has been a big source of debate in the biomedical ethics community about consent and racial disparities in healthcare.