Answer:
cytosol (intracellular fluid), interstitial fluid, plasma
Answer: Protons contribute towards making ATP by producing proton-motive force that provides energy for ATP synthesis.
Explanation: In the respiratory chain, the transfer of electrons from one complex to another is accompanied by pumping of protons out of the matrix. This creates a difference in proton concentration and separation of charge across the mitochondrial inner membrane. The electrochemical energy inherent in this difference in proton concentration called proton-motive force is used to drive ATP synthesis as protons flow back passively into the matrix through a proton pore.
Hello. This question is incomplete. The full question is:
"You determine that you have only 3 copies left of an important DNA fragment, so you decide to amplify it. Using flanking primers, how many PCR cycles would you have to run to generate over one billion (10^9) copies of the fragment?
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Answer:
Approximately 29 cycles of PCR would be required.
Explanation:
As you may already know, PCR is a technique used in molecular biology that allows part of a DNA molecule to be recycled into millions of copies.
PCR allows this replication to be done through cycles. Each PCR cycle lasts about 2 minutes and allows the DNA molecule to undergo the separation of the strands, the binding of the primers and the synthesis of new DNA strands through DNA polymerase. This cycle is usually repeated 32 times, but this number may change depending on the number of copies the researcher thinks is necessary.
In this case, if a researcher wants 10 ^ 9 copies of DNA, he must do the following calculation to find the number of PCR cycles needed:
32 ^ x = 10 ^ 9
x = 28.3 = approximately 29 cycles.
Answer and explanation;
The hydrophobic effect is the observed tendency of non-polar substances to aggregate in an aqueous solution and exclude water molecules. Hydrophobic interaction is mostly an entropic effect originating from the disruption of highly dynamic hydrogen bonds between molecules of liquid water by the non-polar solute.
Introduction of such a non-hydrogen bonding surface into water causes disruption of the hydrogen bonding network between water molecules.
Water dissolves molecules (proteins and nucleic acids) that display on their solvent-accessible surfaces groups that can h-bond [hydroxyl, carbonyl, ester groups; they're polar uncharged...also charged groups; protonated amines, carboxylates and phosphate ethers.
Molecules with internal h-bonds dissolve in water, some/all internal h-bonds may be in dynamic exchange with h-bonds in water
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Polar nature of water contributes to ability to dissolve non-ionic polar organic molecules, such as phenols, esters, amides; have large dipole moments and interaction with water dipole promotes solubility in water.
