Question

Describe at least two other experiments that could be done to verify that chromosomal gene editing occurred in the bacteria.

Answer 1

The two ways by which we can detect that gene editing have occurred in the bacteria are;

A) WESTERN BLOT; Here,the gene that is edited will not be able to synthesize the protein.With this,if we do western against the already edited protein,no band will eventually appear in the western blot,and this indicates/confers that the gene have been edited.

B) PCR FOLLOWED BY SEQUENCING; IN PCR,there is amplification of the region with the use of flanking end primers that is edited.

However,mean primers will actually be used in a way that one primer is upstream to site of editing and the other primer to the downstream.

From this stage,the PCR product that is amplified will be sent for sequencing,and this sequency result will further confirm if the gene has been edited or not edited.

Restriction enzyme can also be used if the place where the gene has been edited is known.

If actually the gene has been edited,the nucleotide sequence will get changed.Having to choose such enzyme that cut only one's wide type wild type DNA but not actually gene that is edited,the result will further be compared inorder to confirm whether the gene has been edited or not.