In step 2: Denaturation of the double-strand occurs.
In step 3: Annealing of the primer to the single strands.
In step 4: Extension or elongation takes place in this step.
Explanation:
In the PCR program an enzyme Taq Polymerase is used because it can withstand high temperature without altering its functions.
PCR is required for the amplification of DNA into multiple copies for experimental purpose. The artificial environment is created to form new DNA molecules from the sample in question.
The first step in replication is the opening of the double helix which is done by temperature treatment in PCR. The temperature would be 90 degrees for some 30 sec to two minutes.
The next step of primer annealing would be done at 52 degrees, this is the primer melting temperature.
The elongation of the DNA strand to be synthesized will take place at 72 degrees as Taq Polymerase can withstand that temperature.
Nearly one million copies of DNA will be made after 30 cycles of PCR.
PCR products can be stored at 4 degrees for some two months.
Answer:
Plants grow well at optimum temperature. When the temperature goes too high, plants growth is negatively effected. During high temperature of the surrounding, photosynthesis reaction slow down while respiration rate increases. Due to increase in respiration rate, the growth and development of plants is stopped because more food is utilized for energy production as compared to food production.
Based on selective permeability, if the pores in the dialysis tubing were too small to allow iodine potassium to pass through, the liquid in the tubing would not turn black.
<h3>What is Selective permeability</h3>
Selective permeability refers to the ability of a membrane to allow some molecules to pass through it but no others.
In the dialysis tubing, selective permeability is used to remove some substances but not others.
The selective permeability of the dialysis tubing depends on the pore size.
When a dialysis tubing containing starch solution was dipped into a beaker containing iodine, the solution in the tubing turned black due to the ability of iodine molecules to pass through the membrane.
However, if the pores in the dialysis tubing were too small to allow iodine potassium to pass through, the liquid in the tubing would not turn black.
Learn more about selective permeability at: selective permeability
Yes
It does
Or so that is what we are taught
