Answer:
The plasmid must express a gene for ampicillin resistance (the protein product of the <em>bla</em> gene codes for beta-lactamase, the protein that breaks down ampicillin). The colonies on the ampicillin plate are antibiotic resistant. This means that they have taken up the transformed plasmids expressing both the <em>bla</em> gene and the GFP gene.
Explanation:
The transformation involved the genetic modification of a plasmid to incorporate the gene encoding the green fluorescent protein (GFP) from jelly fish. GFP makes cells glow under UV light.
In genetic engineering, scientists use antibiotic resistance as markers to indicate cells that have been transformed. By incorporating an antibiotic resistance gene such as <em>bla</em> into the vector (plasmid) and then growing the cells in antibiotic media, scientists determine which colonies have taken up the plasmid. Therefore, if the cells survive, this means that they contain the plasmid with antibiotic resistance gene as well as the GFP gene.
Answer:
Restriction enzyme cuts out gene of interest, Gene is inserted into plasmid (circular) DNA of bacteria using same restriction enzymes, DNA ligase seals DNA together, Plasmid is inserted into bacteria (transformation)
The goal for both is to Change the DNA of the person with the genetic disease
Answer:
Eukaryotic cells, like plant and animal cells, have membrane-bound organelles like the <u>nucleus and mitochondria.</u>
Explanation:
The answer I came up with is Echinoderms and Chordates.
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