Question

The hypothesis was that all three segments of the regulatory region are required for highest expression of the hoxd13 gene. is this hypothesis supported by the results? the hypothesis was that all three segments of the regulatory region are required for highest expression of the hoxd13 gene. is this hypothesis supported by the results? no; they did not delete the promoter, so the gene could still be expressed even without the segments. no; even when segments were deleted, the hoxd13 gene was still being expressed. yes; when any of the segments were deleted, the expression level dropped to less than 100% of the control. yes; when all three segments were present, the expression level was at 100%.

Answer 1

Attached is the image concerning the HOXD13 gene along with the regulatory and promotor regions of the gene.

The answer will be "yes; when any of the segments of deleted, the expression level dropped to less than 100% of the control."

The experiment comprises of testing the expression of the HOXD13 mRNA from the gene by deleting segments of the regulatory regions. In the event of deletion of one segment (segment C), the mRNA expression dropped to around 60% of the control. When two segments are deleted (segments B and C), the mRNA expression dropped to less then 40% of the control. When all segments are deleted, the mRNA expression was almost to 0%.