Question

Place the steps of constructing a genomic library in order. I. Digest phage with restriction enzyme. II. Lyse cells of interest and precipitate DNA. III. Introduce constructs via transformation into competent E. coli cells. IV. Perform ligation reaction with genome fragments and phage. V. Digest genomic DNA with restriction enzyme.g

Answer 1

Answer:

The correct answer is -

II: lyse cells of interest, precipitate DNA

V. Digest (cut) genomic DNA with restriction enzyme

I. Digest (cut) phage (chromosome) with restriction enzyme so can ligate in the gene in next step!!

IV. Perform ligation (glue plasmid in!) reaction with genome fragments and phage.

III Introduce constructs via transformation into competent E. coli cells.

Explanation:

The total collection of the genomic DNA of an organism is known as the genomic library of the particular organism. In this library, the genomic DNA is inserted and stored in identical vectors.

The genomic library construction involved some major steps that are:

- Extract and purify DNA - lysing and separating the desired DNA of interest.

- Digest the DNA with a restriction enzyme - breaking or digesting DNA into fragments with restriction enzymes

- cut phage (chromosome) with restriction enzyme

- Insert the fragments of DNA into vectors

- introduce these recombinant a host bacterium by transformation, creating a DNA library.