1answer.
Ask question
Login Signup
Ask question
All categories
  • English
  • Mathematics
  • Social Studies
  • Business
  • History
  • Health
  • Geography
  • Biology
  • Physics
  • Chemistry
  • Computers and Technology
  • Arts
  • World Languages
  • Spanish
  • French
  • German
  • Advanced Placement (AP)
  • SAT
  • Medicine
  • Law
  • Engineering
ella [17]
3 years ago
5

Place the steps of constructing a genomic library in order. I. Digest phage with restriction enzyme. II. Lyse cells of interest

and precipitate DNA. III. Introduce constructs via transformation into competent E. coli cells. IV. Perform ligation reaction with genome fragments and phage. V. Digest genomic DNA with restriction enzyme.g
Biology
1 answer:
DerKrebs [107]3 years ago
7 0

Answer:

The correct answer is -

II: lyse cells of interest, precipitate DNA

V. Digest (cut) genomic DNA with restriction enzyme

I. Digest (cut) phage (chromosome) with restriction enzyme so can ligate in the gene in next step!!

IV. Perform ligation (glue plasmid in!) reaction with genome fragments and phage.

III Introduce constructs via transformation into competent E. coli cells.

Explanation:

The total collection of the genomic DNA of an organism is known as the genomic library of the particular organism. In this library, the genomic DNA is inserted and stored in identical vectors.

The genomic library construction involved some major steps that are:

- Extract and purify DNA - lysing and separating the desired DNA of interest.

- Digest the DNA with a restriction enzyme - breaking or digesting DNA into fragments with restriction enzymes

- cut phage (chromosome) with restriction enzyme

- Insert the fragments of DNA into vectors

- introduce these recombinant a host bacterium by transformation, creating a DNA library.

You might be interested in
Suppose you are studying coyotes and wolves, and you determine that wolves are experiencing a reduction in their population that
PolarNik [594]
<span>If one determines that wolves are experiencing a reduction in their population that can be attributed to a virus the coyotes carry that is more harmful in wolves. this is an example of apparent competition.</span>
4 0
2 years ago
What is mRNA?
Dovator [93]

Answer:

C

Explanation:

3 0
2 years ago
Scientists sink concrete blocks in oceans to create artificial coral reefs. Which successive event most likely occurs first on t
aliina [53]

Answer:P

Explanation:got it right

8 0
3 years ago
Types of soil erosion
Daniel [21]
Soil erosion can be caused by acid rain, mining, and water
6 0
3 years ago
Now follow these directions to classify the insect specimens. Start by putting on your gloves and goggles. Then, using the force
dybincka [34]

The dichotomous key is an important tool to identify the species of living beings, with this tool it was possible to identify the proposed species, however, to understand this question, we need to know that.......

<h3>Dichotomous Key for Insects</h3><h3 />

This dichotomous key is a simplification and is used to find the order of most insects. But as in biology there is always someone or something to deny us, there are insects that defy this order 1.

Start by choosing between one of the two alternatives of option number 1 and go to the indicated number:

 

  1. - Obvious, membranous wings, sometimes covered with scales or fur:  - Wings absent or forewings thick and hiding membranous hindwings: 15
  2. Wings covered with small scales and proboscis-shaped mouthparts: LEPIDOPTERA
    - Wings not covered with scales, usually translucent. Mouthpiece is not a proboscis: 3
  3. - With a pair of wings: DIPTERA
    - With two pairs of wings: 4
  4. - Long, narrow wings, fringed with fur. Size 5 mm or less: THYSANOPTERA
    - Wings not narrow and fringed: 5
  5. - Abdomen with 2 or 3 long filaments. Small hindwings: EPHEMEROPTERA
    - Abdomen with short filaments or none. Full-sized hindwings: 6
  6. - Forewings clearly longer and with a larger area than the hind wings: - Four forewings as long as, or nearly as long as the hind wings and with a similar or smaller area: 10
  7. - Wings hairy, opaque, with long palps antennae as long as the body or longer: TRICHOPTERA
    - Transparent or translucent wings, hairless, palps short or absent, antennae shorter than the body: 8
  8. - Tarsus with 5 segments, usually with a wasp or bee face: HYMENOPTERA
    - Tarsus with 2 or 3 segments, without a bee or wasp face: 9
  9. - Sucking mouthpiece, nozzle starting at the back of the head. Small to large: HOMOPTERA
    - Chewing mouthpiece, without beak, length 7 mm or less: PSOCOPTERA
  10. - Head extended ventrally forming beak-shaped structure: MECOPTERA
    - Head not extended ventrally: 11
  11. - Very short antennae with thick fur and large eyes: ODONATA
    - Antennas not so short. Moderate to small eyes: 12
  12. - Hindwings wider than forewings. Cerci present: PLECOPTERA
    - Hindwings as wide or almost as wide as forewings.  

With this identification key we can taxonomically classify the 10 insects contained in the question, and with that we can discover the species we are studying.

Taxonomy is an important tool to better understand the species of living beings, their species diversity and their role in ecology.

Learn more about species diversity in brainly.com/question/13259455?referrer=searchResults

6 0
2 years ago
Other questions:
  • Q1.16 Suppose an entire village from a very wet region of Africa escaped mosquitoes by moving to an unpopulated area in a dry re
    11·1 answer
  • 12.
    9·1 answer
  • The preoperational belief that all things are alive and have intentions, motivations, and feelings is called?
    11·1 answer
  • Everyone lives in a watershed explain two procotions people can take in their yard and driveway to help protect their local wate
    15·1 answer
  • Which of the following mutations would most likely be identified as a chromosomal translocation
    14·1 answer
  • A/Producer
    13·1 answer
  • 13. Heme is an achiral molecule that binds to the protein myoglobin. However, a 30 micromolar solution of myoglobin (containing
    6·1 answer
  • Amino acid is to protein as
    12·2 answers
  • Fun fact for today<br> Chickens are the closest living relatives to the T-Rex
    6·2 answers
  • Why ureolytic bacteria use urea? And why they produce CaCO3?
    10·2 answers
Add answer
Login
Not registered? Fast signup
Signup
Login Signup
Ask question!