A technique used in determining the protein binding region in dna (e.g, binding region of RNA polymerase) is called DNA footprinting
One technique used to determine protein binding regions (eg, RNA polymerase binding regions) of DNA is called DNA footprinting. A DNA footprint shows where a protein binds to a DNA molecule. In this technique, a pure DNA fragment labeled with 32 P at one end is first isolated. A trace amount of DNA endonuclease is then added to the mixture of core protein and radiolabeled DNA. RNA sequencing (RNA-Seq) harnesses the power of high-throughput sequencing methods to gain insight into the cellular transcriptome. Compared to previous Sanger sequencing and microarray-based methods, RNA-Seq offers much broader coverage and resolution of the dynamic nature of the transcriptome.
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Answer:
Explanation:
Bacteriorhodopsin is one of the simplest protein from a family of a protein called opsin which is present in photosynthetic bacteria, algae and few fungi.
The bacterio-rhodopsin acts as an active light-gated proton pump which can pump the protons against their concentration gradient and then establishes concentration gradient across the membrane.
When the photosynthetic bacteria gets exposed to the light, bacteriorhodopsin pumps the proton from the cytosolic side to outside. This establishes electric motive force across the membrane which causes disequilibrium of protons.
The protons move back from the outside of the membrane to inside through H+ATPase which help allow the generation of ATP in the cell.
<h2>Answer is option "C"</h2>
Explanation:
- NAD+ is a significant co-compound for hydride move catalysts fundamental to numerous metabolic procedures including glycolysis, pyruvate dehydrogenase complex, the TCA cycle and oxidative phosphorylation. The catalysts utilizing NAD+ in hydride-move are known as dehydrogenases or oxidoreductases, which catalyze the decrease of NAD+ into NADH
- NADH shaped from glycolysis (by means of the malate-aspartate transport) or the TCA cycle can respond at Complex I, otherwise called the NADH/coenzyme Q reductase in the mitochondrial electron transport chain [28]. Each NADH devoured by the mitochondria brings about the net creation of 3 ATP atoms (Figure 1). The total oxidation of one glucose atom creates 2 NADH reciprocals in cytosol and 8 NADH particles in mitochondria, empowering creation of 30 ATP counterparts from NADH of the aggregate of 36 ATP counterparts got from the entire procedure of catabolizing glucose to CO2 and H2O.
- Hence, the right answer is option C " the available NAD+ would be converted to NADH and glycolysis would stop due to lack of NAD+.
Chlorophyll, Chloroplast and heoglobin <span />
A food web describes the flow<span> of </span>energy<span> and nutrients </span>through<span> an ecosystem, ... Organisms </span>can<span> be organized into trophic levels: primary producer, primary ... In both </span>food webs<span> and </span>food chains<span>, arrows point from an organism that is </span>consumed<span> to ... of trophic </span>energy<span> transfers, the </span>amount of energy<span>remaining in the </span>food chain<span> ...</span>
