<span>Negatively charged R-groups in the primary structure.
This is because nickel acts as a cation, therefore you would be attracting anions, in this case it would be on the proteins that would be binding to the column. Once they bind to the matrix a was buffer of high salts can elute the proteins.</span>
Answer:
Like the human body, the bicycle relys on other things to keep moving. For example, in order for the bicycle to keep moving, the rider needs to have legs and be able to push the pedals foreward. In order to breath, the body needs to have lungs and those lungs depend on the heart to pump blood throughout the body. The lungs rely on the heart and your brething relys on your lungs. Hope this helped, brainliest always appreciated.
Explanation:
