Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
Seismic waves, Outer, Mantle
Explanation:
P-waves vibrate longitudinally to the direction of propagation while S-waves vibrate vertically to direction of propagation. This is why S-waves are called shear waves while P-waves are compressional waves. S-waves are the main cause of destruction in an earthquake and are slower than P-waves.
The wave movement of P-waves allows them to pass through liquids and gases while S-waves cannot. Due to this differential phenomenon of these waves, the reflection of P –waves and S-waves after an earthquake – and as their vibrations are picked up by seismographs around the earth - enabled scientists to determine the properties of the layers of the earth’’s interior.
Because it is polar and it is to large to cross the membrane and because the concentration gradients are to strong so they have to be diffused.
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Answer:
A- Acetylcholine is released by axon terminals of the motor neuron.
Explanation:
The general mechanism of muscle contraction can be explained as follows:
a) The action potential which is generated, travels along the motor nerve to its ends on muscle fiber (or motor end plate) at the neuro-muscular junction.
b) The nerve endings then secrete small amounts of neurotransmitter called acetylcholine.
c) The actylecholine binds to the receptors on the membrane of muscle fiber and opens the voltage gated sodium channels.
d) Opening of these channels leads to flow of large quantities of sodium ions into the muscle fiber which ultimately results in initiation of action potential in muscle fiber.
e) This action potential depolarizes the fiber including the T-tubules, triggering the release of calcium ions from sarcoplasmic reticulum.
f) The calcium ions thus released initiated the process of muscle contraction.
