Answer:
Both
Both
Both
PCR only
Both
Explanation:
PCR and Restriction enzyme digestion: Requires controlled temperature changes using a thermocycler Isolating purified modified plasmid is necessary for analysis
. However this is not always the case with PCR as genomic DNA or DNA fragments can also be used.
PCR and Restriction Enzyme Digestion: Using a different vector plasmid yields a different outcome
. This is because different plasmids have different sequences that can change the size of the amplicon. Also, in the case of Restriction Enzymes, there are different restriction sites. This makes various plasmids useful for tailoring the cutting sites.
PCR and Restriction Enzyme Digestion: Gel electrophoresis is required for analysis. This process is done in PCR to observe the presence and size of an amplicon. This would tell if the PCR was successful or not. It is also wise to use gel electrophoresis to check the status of the Enzyme Digestion. This confirms that the site was cut or the plasmid was unable to be cut.
PCR only: Amplify a specific region of DNA
. This is the purpose of the PCR. If there is to be amplification of a region of a DNA it can be done using a plasmid if it is placed in a competent cell designed for this procedure.
PCR and Enzyme Digestion: Requires the knowledge of predicted DNA fragment size for analysis. Without understanding the size of the amplicon it will be difficult to tell if the PCR was successful or not. Additionally, after the enzyme digestion and Gel Electrophoresis the examination of the size of the "cut" DNA is also important to confirm the right cutting sites.
PCR and Restriction Enzyme Digestion are two important tools used in Molecular biology experiments that can give the scientist an insight on the type of material that they are working with and the way that the experiment is progressing.
