Question

You transformed bacteria with varies plasmids and counted the colonies as well as colony color. All bacteria were plated on media containing ampicillin. See below. Transformed with Colonies observed 1. Plasmid with GFP gene 100 green colonies 2. Plasmid without GFP gene 100 white colonies 3. No plasmid No colonies What conclusions are supported by these results

Answer 1

Answer and Explanation: GFP gene is a gene that encodes a green fluorescent protein. When bacteria and plasmid are combined, the gene is expressed as observed by the results: plasmid with GFP gene produces colonies with green color; while plasmid without GFP produces white colonies.

Ampicillin is an antibiotic, i.e., bacteria is naturally suscetiple to it.

Plasmid has an ampicillin resistant gene. This is demonstrated in the experiment: when there is plasmid, the gene can be expressed, allowing colonies to grow. But, when bacteria is not combined with plasmid, it is not resistant to the antibiotic, so no colonies are formed.