Site-directed mutagenesis (SDM) is a technique widely used in molecular biology in order to generate site-specific, targeted changes in the DNA sequence of a gene of interest. The SDM protocol consists of using a complementary oligonucleotide (primer) that contains the desired mutation, which hybridizes to the target DNA sequence and thus can trigger the desired mutation. Nowadays, the versatile CRISPR-Cas9 genome editing system is the most used technique to produce targeted mutations in any gene of interest.
