In step 2: Denaturation of the double-strand occurs.
In step 3: Annealing of the primer to the single strands.
In step 4: Extension or elongation takes place in this step.
Explanation:
In the PCR program an enzyme Taq Polymerase is used because it can withstand high temperature without altering its functions.
PCR is required for the amplification of DNA into multiple copies for experimental purpose. The artificial environment is created to form new DNA molecules from the sample in question.
The first step in replication is the opening of the double helix which is done by temperature treatment in PCR. The temperature would be 90 degrees for some 30 sec to two minutes.
The next step of primer annealing would be done at 52 degrees, this is the primer melting temperature.
The elongation of the DNA strand to be synthesized will take place at 72 degrees as Taq Polymerase can withstand that temperature.
Nearly one million copies of DNA will be made after 30 cycles of PCR.
PCR products can be stored at 4 degrees for some two months.
Answer:
study a larger sample size than before.
Explanation:
<span>Antibiotic resistance arises due to the evolution of the organisms they are designed to destroy. Subsequent generations of such organisms develop resistance to the antibiotic. Hospitals' overuse of antibiotics quickens the process, as does the administration of antibiotics to feedlot cattle, whose meat is consumed by people.</span>
Answer:
This is called replication.Generally, in an experimental set up experimental findings needed to be repeated continuously until a reasonable similar conclusion is reached.
Scientists needed to carry out similar experimental findings to get a similar results, to arrive at a reasonable conclusion.
It is the stage that is important for them to check the results arrived at.Replication therefore helps to identify any variability in the experimental results,for accurate results.
Explanation:
Answer: birds and the bees/cartoon
Explanation:if they are 13 an up tell them about the birds and the bees but if they younger than that show them a cartoon
