Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
1. Yes
2. No
3. Yes
Explanation:
Yes, the elimination of the animal based foods decreases and reduce chronic diseases in many developed countries such as US and England etc. but meat is also very necessary for animal diet due to the presence of high protein content.
No, USDA does not promote the intake of an animal-based diet. this agency promote the intake of nutritious food in a limited amount so it does not cause any disease. They recommend the eating of Grains, Fruits, Protein, Foods, Vegetables and Dairy products in a limited amount.
Yes, the main cause of obesity in Americans is due to two reasons, first cause is the intake of meat in large amount and second cause is the decline in physical activity.
The success of the patients was due to combined effect of increased amount of fruits/vegetables and plants and the elimination of meats. By using these two element the patient will quickly recovered.
We’re is the picture I need to see the picture
Answer:
In the absence of sunlight the plants which are dependent on sunlight for making food that is photosynthesis would die.
Also the people will suffer as sunlight is a good source of Vitamin D and in absence of sunlight they will not get it so easily.
The evidence would be all the different types of species
