Question

Which of the following is a concern if you forgot to resuspend (mix) culture solutions when performing serial dilution?

Answer 1

Answer:

B) CFU/mL calculations would not be accurate

Explanation:

In microbiology CFU is the acronym for Colony Forming Unit, which indicates the number of bacteria or fungi capable of multiplying under suitable conditions. In this case, CFU/mL indicates how much bacterial or fungal cells, susceptible to reproduction, exist within 1 mL of a culture solution.

When this solution is used, to make a dilution or other laboratory activity, it is important that it be resuspended, that is, mixed, as the bacteria or fungus cells can accumulate in the bottom or surface of the substance, changing the value of CFU/mL and causing inaccurate dilutions if used. When resuspended, the substance presents the appropriate value of CFU/mL, ensuring that the dilution is accurate and effective.