I believe the changed or converted substrate, now the product of the chemical reaction is released from the active site of an enzyme during the chemical reaction.
More bonds are broken than made. This is because the energy will remain in the substance, making it an endothermic reaction.
-A-A-A-T-G-C- because only one base pair is altered by the mutation
My hypothesis will be that the enzyme pH will have a hot temperature and will make small amounts of the enzymes activity because it takes time to process.
Peripheral would be the answer