Which of the following statements is correct regarding engineering maize plants with a herbicide-resistance gene using Agrobacte
rium-mediated plant transformation? a. The Ti-plasmid used for this experiment must contain tumor-inducing genes.
b. The herbicide-resistance gene is cloned into the T-DNA region of the Ti-plasmid.
c. The herbicide-resistance gene is cloned into a region outside of the T-DNA in the Ti-plasmid.
d. A guide RNA is used to transfer T-DNA from Agrobacterium to the plant cell.
e. The whole Ti-plasmid containing the herbicide-resistance gene is inserted into the maize genome after infection with Agrobacterium
b. The herbicide-resistance gene is cloned into the T-DNA region of the Ti-plasmid.
Explanation:
Ti-plasmid has the ability to introduce part of its DNA (t-DNA) into the genome of host plants. This is very important for the relationship of genetic manipulation in maize plants, with the aim of inducing a gene that promotes herbicide resistance. In addition, T-DNA has many genes that code for the production of plant growth phytohormones, which is extremely important for the cloning process to work. In this case, we can assume that the correct answer for yours is the letter B.
