Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer;
- 40% ; 20%
Obese workers spend nearly 40% more on health care than normal weight adults, adds about 20 percent to healthcare spending.
Explanation;
-Obesity is one of the biggest drivers of preventable chronic diseases and healthcare costs in the United States. Obese adults spend 42 percent more on direct healthcare costs than adults who are a healthy weight.
-Costs for patients presenting at emergency rooms with chest pains are 41 percent higher for severely obese patients, 28 percent higher for obese patients and 22 percent higher for overweight patients than for healthy- weight patients.
Answer:
pharynx, larynx, trachea, bronchi and lungs
The respiratory system is what allows us to breathe and exchange carbon dioxide for oxygen. The human respiratory system is a series of organs responsible for taking in oxygen and expelling carbon dioxide. ... The lungs work with the circulatory system to pump oxygen-rich blood to all cells in the body.
The respiratory system does not work alone in transporting oxygen through the body. The respiratory system works directly with the circulatory system to provide oxygen to the body. Oxygen taken in from the respiratory system moves into blood vessels that then circulate oxygen-rich blood to tissues and cells.
Explanation:
Dominate and no know preditors <span />
First one is correct. ...remeber my teacher telling us enzymes never get used
