In microscopy, the term resolution In microscopy, the term resolution refers to the ability to distinguish fine structure and de
tail in a specimen. is only observed in stained specimens. refers to magnification when using the electron microscope. is the same as the total magnification of a specimen. is improved when longer wavelengths of light are employed.
refers to the ability to distinguish fine structure and detail in a specimen
Explanation:
Resolution can be defined as the capacity of a microscope to distinguish detail. Resolution can be measured as the minimum distance between two distinct objects of a specimen which can still be observed as separate entities. Some important factors influencing microscope resolution include 1- objective numerical aperture, which is related to the angles of light that are collected by a lens (can be improved by using immersion oil), 2- degree of aberration correction, 3-magnification (i.e., degree of visual enlargement of an observed object), 4- contrast methodology, etc. In a light microscope, for example, the maximum resolution that can be reached is 200 nanometers (nm), being this limited by the wavelength of light waves due to diffraction, while the maximum magnification is generally x1500.
