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malfutka [58]
2 years ago
12

) Imagine that you are studying a newly discovered bacteria from a hot spring in Yellowstone National Park. You examine the nitr

ogenous bases of this organism, and find that 10% of the nitrogenous bases in its DNA are adenine. What percentage of nucleotides are cytosine
Biology
1 answer:
ikadub [295]2 years ago
5 0

Answer:

Cytosine- 40%

Explanation:

DNA is the genetic material in virtually all living organisms. It is a molecule made up of long chains of nucleotides, which are its building block. Four types of nucleotides make up the DNA molecule viz: Adenine (A), Thymine (T), Guanine (G), and Cytosine (C).

According to Erwin Chargaff in his rule regarding DNA composition, he stated that in a DNA molecule, the amount of adenine must equal thymine while that of guanine must equal cytosine. In a nutshell, the total of all the base pair must equally be 100% i.e. (A + T) + (G + C) = 100%.

In this case where a newly discovered bacteria from a hot spring in Yellowstone National Park was found to contain 10% of adenine in its DNA. Since Adenine is 10%, Thymine base must also be 10% in accordance to Chargaff's rule.

Hence, 10% + 10% + (G + C) = 100%.

20% + G + C = 100%

G + C = 100 - 20

G + C = 80%

Since, G and C must be equal, then G = C = 80% ÷ 2 = 40%. This means that the percentage of cytosine will be 40%.

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Hey there!


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malfutka [58]

Answer: the cfu/g Gram-negative bacteria in the fecal sample is C = 3.0 × 10^3

Explanation:

We know that; Gram negative bacteria looks  pale reddish in color under a light microscope from Gram staining.

therefore

There are 30 red bacterial colonies counted.

1 mL of from tube 1 was removed and added to tube with 99 mL saline (tube 2) dilution is 1/100.

transferred volume  into the plate is 1 mL.

Now, we have to determine the cfu/g Gram-negative bacteria in the fecal sample

Formula to calculate CFU/g bacteria in fecal sample is expressed as;

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where C is concentration (CFU/g) , n is number of colonies , s is volume transferred to plate , d is dilution factor.

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C = 3000

C = 3.0 × 10^3

THERFERE, the cfu/g Gram-negative bacteria in the fecal sample is C = 3.0 × 10^3

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