Aspartate + a- keto acid >> Oxaloacetate + a amino acid
This reaction is occur because the a- keto acid in the equation can come from any amino acid, the 15 N from aspartate is rapidly transferred to other amino acids.
15N is symbol use as the isotope of nitrogen with mass number 15. comprises of 0.4 % of stable nitrogen found, so the relative abundance of each in amino acid is simply a reflection of how much is found in nature in general.
This isotopes is enrichment of the labelled amino acids was determined following a previously developed procedure comprising by determination of the spectral purity of the selected natural abundance amino acids.
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Answer:
Explanation:
1. Visual design
Users can be distracted by the lack of visual design on a prototype because wireframes and other low-fidelity prototypes are very basic. This can cause users to comment on the lack of design and colour and distract both themselves and the researcher from the true goals of the project. The extent of this challenge depends on the level of detail within the prototype.
How to get around this: Ensure the user is aware at the start of a session that the website they are about to view is at an early stage of development and so does not look and feel like they may expect. The research may need to be explicit with some users and point out it is not the visual design that we are interested in for today.
2. Partial journeys
Prototypes often cover only partial user journeys, meaning that users may have to be dropped into a journey at a specific point and may lose the context of the overall task or what they would be coming on the site to do.
How to get around this: As well as creating tasks which set the context, consider including some time at the beginning of the session for users to explore the prototype as they would normally do on that website/app, without giving them long enough to discover the prototype journeys. Introductory questions can also be asked at the start of the session to position the user in the right frame of mind for what the prototype will allow them to do, therefore helping to provide some context alongside the task wording.
