Confirm that the sample has both helicase and primase activities, but not the ability to synthesize DNA
- The replisome is a multiprotein-RNA complex that is required for the replication of DNA. The replisome consists of different proteins which include:
1. Helicase enzymes that unwind and separate the DNA strand
2. Replication factor C (RFC), a DNA-dependent ATP that acts as an activator of DNA polymerase.
3. Proliferating cell nuclear antigen (PCNA)
4. DNA gyrase/topoisomerase that cuts and reseals DNA strands, which is essential for DNA synthesis.
5. Primase enzyme that synthesizes short RNA fragments called primers.
6. DNA polymerase III, an enzyme that catalyzes the polymerization of deoxyribonucleotides into a DNA strand.
7. DNA ligases enzymes that form phosphodiester bonds at a single-strand break in DNA
- A primosome is a multiprotein complex responsible for creating RNA primers on single-stranded DNA (ssDNA) during the replication process.
- The primosome is composed of different primase and helicase enzymes. In<em> Escherichia coli</em>, it consists of PriA helicase, PriB, PriC, DnaB helicase, DnaG (encoded by the dnaG gene), DnaC and DnaT primase.
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Yes Ligase is involved in dna
Answer: energy can be cycled in two directions
Explanation: The arrows show the direction of matter and energy flow and should be two
Plants provide oxygen for the planet and organisms, food/nutrients, and habitats for animals
The reaction which connects monomers (polymerization) is dehydration synthesis.
The name tells you about the nature of the reaction - dehydration means 'loss of water', correct? When two biological monomers connect, one gives up a hydrogen ion (H+), and the other gives up a hydroxyl group; (OH-). They combine to form H2O - water.
