Answer:
D.
Explanation: Ecology: relation of organisms with one another or their surroundings.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
NAD is a coenzyme found in all cells. It consists of two nucleotides linked through their phosphate groups with a nucleotide that contains an adenosine ring and another that contains nicotinamide.
Explanation:
In metabolism, NAD participates in oxidation reduction reactions. This coenzyme is found in two forms in the cells: NAD and NADH. NAD accepts electrons from other molecules and is reduced, forming NADH, which you can use as a reducing agent to donate electrons. These electron transfer reactions are the main function of the NAD.
In living organisms, NAD can be synthesized from scratch from the amino acids tryptophan or aspartic acid. Some NADs are found in nicotinamide adenine dinucleotide phosphate (NADP), whose chemistry is similar to NAD, although it has different functions in metabolism.
Answer:
This is the site of gaseous exchange
Explanation:
Gaseous exchange is the mechanism by which oxygen is substituted for CO₂ in the body. CO₂ laden blood is carried to the alveoli, which is wrapped with numerous capillary beds and with walls that are thin. When a person inhales, the difference in the partial pressure of the various gases ( oxygen and carbon dioxide) causes them t move down their concentration gradients, with CO₂ diffusion into the lungs from red blood cells, and oxygen binding to the haemoglobin in the red blood cells, after which they are carried to all body tissues for cellular respiration. During exhalation, the CO₂ in the lungs is expelled to the exterior and the process repeats.
