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zhannawk [14.2K]
3 years ago
5

In the SDS-PAGE (sodium dodecylsulfate - polyacrylamide gel electrophoresis) method, separation takes place on the basis of:____

_________
a. charge only, because all particles have different charges, but the same mass.
b. the sieving action of the gel, because all particles have the same charge, but different masses.
c. the sieving action of the gel, because all particles have approximately the same charge/mass ratio, but different masses.
d. the chemical nature of the buffer used as the electrolyte.
Biology
1 answer:
Vera_Pavlovna [14]3 years ago
8 0

Answer:

B

Explanation:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a method of separating molecules based on the difference of their molecular weight.

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Explain cancer in terms of the cell cycle
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hey mate this is your answer

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Please help its due in a hour!!!!!
garri49 [273]

Answer:

1. The difference between the normal hemoglobin protein DNA sequence and the sickle cell hemoglobin DNA sequence is a base to base shift, in this case adenine (GAG) to thymine (GTG).

2. The difference affects the amino acid sequence of the protein by replacing glutamic acid (Glu) with valine (Val).

Explanation:

In sickle cell anemia, a change in the DNA nucleotide sequence is observed, where adenine is substituted by thymine, whose expression is the change in the amino acid sequence of globine β, incorporating valine instead of glutamic acid. This represents a molecular mutation - point mutation - by subtitution, which corresponds to missense mutation.

<u>Normal hemoglobin protein in a RBC</u>

DNA                 CTG ACT CCT GAG GAG AAG TCT

Amino acids     Leu  Thr   Pro   Glu   Glu   Lys   Ser

<u>Sickle cell hemoglobin protein in a RBC</u>

DNA                 CTG ACT CCT <em>GTG</em> GAG AAG TCT

Amino acids     Leu  Thr   Pro   <em>Val</em>   Glu   Lys   Ser

When GAG is transcribed to mRNA, the CUC codon is obtained, which codes for glutamic acid. Thymine substitution causes the DNA sequence to change to GTG, which is transcribed as CAC, the codon that encodes the amino acid valine. The <u>change from glutamic acid to valine in β-globin causes an altered hemoglobin, giving the abnormal erythrocytes observed in sickle cell disease</u>.

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Without the keystone species, the rocky inter-tidal ecosystem would be dramatically different or cease to exist altogether

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Keystone species have low functional redundancy. This means that if the species were to disappear from the ecosystem, no other species would be able to fill its ecological niche.

For instance; the starfish Pisaster ochraceus is a keystone species in the rocky marine inter-tidal communities off the northwest coast of North America. It feeds on the mussel Mytilus californianus and is responsible for maintaining much of the local diversity of species within certain communities.

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They disguise themselves as white blood cells
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They can both be used to identify organisms. Also, the phenotype of an organism is different depending on the genotype of the organism, making them relate to one another.

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