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zhannawk [14.2K]
3 years ago
5

In the SDS-PAGE (sodium dodecylsulfate - polyacrylamide gel electrophoresis) method, separation takes place on the basis of:____

_________
a. charge only, because all particles have different charges, but the same mass.
b. the sieving action of the gel, because all particles have the same charge, but different masses.
c. the sieving action of the gel, because all particles have approximately the same charge/mass ratio, but different masses.
d. the chemical nature of the buffer used as the electrolyte.
Biology
1 answer:
Vera_Pavlovna [14]3 years ago
8 0

Answer:

B

Explanation:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is a method of separating molecules based on the difference of their molecular weight.

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Answer:

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Acute kidney injury is when there is sudden and often reversible loss of renal function which develops over days or weeks and is usually accompanied by a reduction in urine output.

There are many causes of AKI, which are classified as prerenal, renal and post renal. In prerenal AKI, the kidneys gets damaged as a result of hypoperfusion leading to acute tubular necrsosis. Common examples are severe dehydration( like in this patient from vomiting), cardiac failure, sepsis, blood loss etc. Renal AKI is usually cause by direct insult to the kidneys which can result from drugs, toxins, inflammatory conditions etc. Post renal AKI occurs when there is obstruction to urine flow at any point from the tubule to the urethra e.g in BPH, stones, strictures etc.

In Prerenal AKI, it leads to acute tubular necrosis. Histologically, the kidney show inflammatory changes, focal breaks in the tubular basement membrane and interstitial oedema.

Chronic kidney disease(CKD) refers to an irreversible deterioration in renal function which develops over a period of years.initially, it is manifestation only as biochemical abnormality but eventually, loss of the excretory, metabolic and endocrine functions of the kidneys leads to clinical symptoms and signs of renal failure.

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