Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
The answer is confirmation bias. This is the tendency to look for evidence that will confirm ones hypothesis and ignoring anything else that does not lead to the hypothesis. In this situation confirmation bias is evident due to the fact that you look for anything that confirms your hypothesis and ignore anything else that does not point to drug abuse.
Either an echo or a reverberation.
Answer:
This quote is by Immanuel Kant and is meant to defend animals from the cruel actions of men.
Explanation:
German Immanuel Kant was one of the most influential philosophers of all time. <u>Kant opposed Utilitarianism</u>, an ethical theory that reduced the rights or wrongs of an action to how positive or negative the outcome of said action was, which could lead to vile actions since nothing was really wrong unless it had bad consequences.
To Kant, animals were deserving of good treatment, and he believed that a man who is cruel to animals is also cruel to other men.
