A plasmid that encodes resistance to ampicillin and tetracycline is digested with the restriction enzyme PstI, which cuts the pl
asmid at a single site in the ampicillin-resistance gene. The DNA is then annealed with a PstI digest of human DNA, ligated, and used to transform E. coli cells. What antibiotic-resistance phenotype(s) (characteristics) will be found on the plate
A plasmid is a small circular DNA molecule found in bacteria that is separate from the bacterial chromosome and replicates independently of it. They encode for certain genes that play a key role in antibiotic resistance. <u>Restriction enzymes are endonucleases that catalyze the cleavage of phosphodiester bonds in different regions located within a DNA strand</u>. PstI is an example of an endonuclease, it is a type II restriction enzyme produced by the microorganism <em>Providencia stuartii</em> that possesses a restriction target in double-stranded DNA dependent on an unmethylated, palindromic, asymmetric sequence, and in this example, it cuts the plasmid at a single site in the ampicillin-resistance gene. After that, the DNA is annealed with another fragment of DNA and this new recombinant molecule is used to transform <em>E. coli</em> cells. Transformation is a key step in DNA cloning because it occurs after restriction enzyme digestion and ligation treatments and transfers newly made plasmids into bacteria. <u>So these bacterias will have a new fragment of DNA, which still has a tetracycline resistance gene but it no longer has the ampicillin resistance gene because it was disrupted by the restiction enzyme</u>. Thereby, the antibiotic resistance phenotype is the tetracycline resistace.
The answer is C. This is correct because if the tree is near the equator, it will constantly be hot nearly everyday, because the Sun points straight at the equator. Because there is only one temperature, the tree doesn’t have to worry about dealing with the cold, only the heat.
People breathe oxygen in and carbon dioxide out, but for a plant to make carbohydrates, the leaves take in carbon dioxide through the stomata on the leaf surfaces. From the stomata, the mesophyll cells use the carbon dioxide to combine it with water and light for photosynthesis.
