Answer:
The impact of murals: the process and the paintings.
In Texas, this region is divided into three distinct sub-regions: the High Plains, the Edwards Plateau and the Llano Basin.
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There are dark regions with numerous craters called impact craters and they cover one-third of the moon. The remainder of the moon is covered by lighter regions which have many ridges and grooves . The ridges are as high as 2000ft and stretch for thousands of miles.</span>
The 3 main regions of the brain are the cerebrum, cerebellum, brainstem.
Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
