The restriction endonuclease EcoRI cleaves the DNA sequence 5'-GAATTC-3' in such a way as to leave a 5' overhang, which is often referred to as a "sticky end". To 5' overhang with the complementary sequence this sticky end adhere.
- The palindrome EcoRI restriction site breaks DNA after G and creates sticky ends with AATT. G/corresponding AATTC's DNA sequence is CTTAA/G, where "/" designates the location of the peptide bond break.
- When the ssDNA sequence 5'-GAATTC'-3 is recognised by the restriction endonuclease EcoRI, a single-strand cut is made between the G and A nucleotides.
- At the ultimate end of double-stranded DNA, an overhang is single-stranded DNA. This can occur on either strand, and if it does, there will either be a 5' overhang on the 5' end of the strand or a 3' overhang on the 3' end of the strand.
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Answer:
EtBr inserts between the stacked bases in the DNA double helix.
Explanation:
- EtBr is used for visualizing DNA bands as it fluoresces under the UV illumination.
- EtBr is an aromatic compound that is capable of inserting itself between the stacked bases of the DNA double helix.
- The hydrophobic environment around the base pairs where the EtBr intercalates is responsible for the fluorescence. As the EtBr molecule intercalates between these base pairs the cation of EtBr sheds the water molecules associated with it and this causes it to fluoresce under UV light as water is a quencher of fluorescence.
