The substage condenser is either incorrectly positioned or the light stop is the wrong size for the objective- or there me be a smudge/mark on the eyepiece
When the organism is preserved in ice.
Isotonic since in a hypertonic solution the cell would shrivel up and in a hypotonic solution it would lyse (burst).
Answer:
Sorry, I'm confused there has to be more to it if there is not then there is no true way for anyone to fill in the blanks. <3
"The rotating part of the microscope at the bottom of the body tube; it holds the objectives." -utahscience.oremjr.alpine.k12.ut.us