Question

Which far-field imaging methods can extend the diffraction limited resolution to a smaller value?

Answer 1

Answer:

Deterministic super-resolution: The most commonly used emitters in biological microscopy, fluorophores, show a nonlinear response to excitation, and this nonlinear response can be exploited to enhance resolution, such as STED, GSD, RESOLFT and SSIM.

Stochastic super-resolution: The chemical complexity of many molecular light sources gives them a complex temporal behavior, which can be used to make several close-by fluorophores emit light at separate times and thereby become resolvable in time, such as Super-resolution optical fluctuation imaging (SOFI) and all single-molecule localization methods (SMLM) such as SPDM, SPDMphymod, PALM, FPALM, STORM and dSTORM.

Explanation:

https://www.creative-biostructure.com/Super-resolution-Microscopy-Service-590.htm