I believe the answer is b
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During DNA Replication, the DNA molecule unzips itself so that there are two free strands of DNA. Both of those strands then create new complementary strands of DNA. Thus every subsequent generation of DNA uses one of the parent cell's strand of DNA as a template, which is why DNA replication is said to be semi-conservative. Hope this helped!
A karyotype shows the _46_ chromosomes sorted and isolated from a cell in _Metaphase_