Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Cytokinesis is the point at which a cell with duplicated genetic material becomes two daughter cells with identical DNA.
Heat can be transferred from one object to another since, If there is a temperature difference between two systems, heat will always find a way to transfer from the higher to the lower system.
Kinetic energy can be transferred from one object to another, when two objects crash. One example of kinetic energy being transferred from one object to another would be a collision of pool balls, Since one ball would hit another causing it to move. Also Kinetic Energy is, “The <u>energy</u> of motion, observable as the movement of an object, particle, or set of particles. Any object in motion is using kinetic energy: a person walking, a thrown baseball, a crumb falling from a table, and a charged particle in an electric field are all examples of kinetic energy at work.”
And last but not least, Thermal Energy which, is often referred to as heat. The thermal energy of matter depends on how fast the atoms or molecules are moving. The faster they are moving, the more thermal energy they possess. Therefore, the temperature of the matter would be higher. Thermal energy is a form of kinetic energy. One example of Thermal energy being transferred from one object to another is, Thermal energy from a hot stove is transferred to a metal pot and causes the water molecules to move faster increasing the temperature of the water. Fun fact; Thermal Energy can be transferred in three ways known as, Conduction, Convention, Radiation.
Answer:
A. parts A and B
Explanation:
A is the filament and B is the anther
The answer is B
topsoil<span>. The potting soil</span>, is use for cultivating floors, grasses and
vegetables in a handle or container. <span>The potting soil</span><span> available in trades is sterilized in order to avoid
the spread of bad grasses and diseases transmitted by the plants.</span>
