Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
The populations of the species there will go extinct
rDNA is using genetic cloning to form DNA molecules from different species. in this example you can make the hormone insulin by getting some of the components from one organism and getting the remainder from another organism. Specifically, Insulin has 2 amine chains, so you can get 1 amine chain from one organism, and the other from a different one. Then you can combine them and you'll get insulin.
<em><u>T</u></em><em><u>h</u></em><em><u>e</u></em><em><u> </u></em><em><u>mutation</u></em><em><u> </u></em><em><u>Mutations are changes to an organism's DNA and are an important driver of diversity in</u><u> </u><u>populations.</u><u> </u><u>This mutation has introduce a new allele into the population that increases genetic variation and may be passed on to the next generation.</u></em>
<em><u>hope</u></em><em><u> </u></em><em><u>it</u></em><em><u> </u></em><em><u>helps</u></em><em><u>.</u></em>
