There are two types of polar climate: ET, or tundra climate; and EF, or ice cap climate. A tundra climate is characterized by having at least one month whose average temperature is above 0 °C (32 °F), while an ice cap climate has no months above 0 °C (32 °F).
Number 1 ,3 and 4 are the answers
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Microinjection is a simple mechanical process usually involving an inverted microscope with a magnification power of around 200x (though sometimes it is performed using a dissecting stereo microscope at 40–50x or a traditional compound upright microscope at similar power to an inverted model).
Movement of lithospheric plates created isolation between populations and with isolation comes different mutations and lineages which is how evolution occurs. (This is called allopatric speciation!).