the correct answer is A- Reproductive isolation occurred through behavioral isolation
A process of formation of a new species from an existing species is called speciation. New species may formed due to geographical or reproductive barriers which isolates the members of a population of a species to form a new species.
In the given question, speciation in In the leopard frogs occurred due to a particular or specific call given by males to the females. The courtship call lead to reproductive isolation due to change in behavior. Such type of behavioral change will help in recognizing mates of the same species and this will act as a reproductive barrier among the other related species.
the insertion on removal of mutations are dangerous in nature. the process is basically just one large gamble to see what it does. and even though we have a decent understanding of the genomes of many animals and plants, we still dont know everything. so if we tamper with certain genes we may cause a evolution or we might kill the subject and the potential for it to procreate.
I believe RNA
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