Answer:
Instantaneous speed is found by taking the absolute value of instantaneous velocity, and it is always positive. Average speed is the total distance traveled divided by elapsed time.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Common examples of this would be if you place your hand in extremely warm or cold water, and an immediate response of pulling out of the water or any fluid, is an example of this extreme response, other responses are what is done in everyday, like being thirsty or hungry etc.
These behaviours are shared by all organisms even the most simple ones.
<span>Rganelles is a term that used by it means - the component in cell as example nucleus,cell wall,cytoplasm...and many more....that have function in cell</span>
