Answer: CFU/mL = 14200 CFU/mL
Explanation:
Microorganisms are very small and difficult to count directly. Instead, a laboratory technique is used to count CFUs (Colony Formation Units). This involves making dilutions of a microbial suspension and spreading it on thin layers of agar containing nutrients for growth in Petri dishes also called agar plates. After incubation under ideal conditions, some plates are completely covered with microbial growth; some have little or no growth; and some are covered with individual isolated "spots", which are microbial colonies. Since a colony may consist of a single organism or a group of microbes, the dots represent a CFU.
To calculate the viable count of the stock culture, you have to count the number of colonies, multiply by the dilution and the result will be the plate count in CFU.
So, the equation is:
CFU/mL = Number of colonies / (Volume trasferred to plate x dilution blank)
100 microliters = 0.1 mililiter
1/10 = 0.1 (dilution factor)
Replacing the information from the question:
CFU/mL = 142 / (0.1 mL x 0.1)
CFU/mL = 14200 CFU/mL
Science is the study of the natural and physical world using a tactful way of denomination called the scientific method.
To connect skeletal muscles to bones.
Answer:
23% im pretty sure that is the answer if i remember right
The reaction of which Missy has experience that is a severe
and life threatening allergic reaction due to penicillin is anaphylactic reaction,
it could be classified as the a serious and life threatening in terms of having
allergic reaction for if not treated immediately, it could cause death to the
individual who has experience this reaction. This could be triggered from
either medications, food in which an individual has consumed and insect stings
such as from bees. Missy’s allergic reaction must have triggered because of the
penicillin that she has consumed where in, she does not know that she is
allergic to the medication, having her to experience a life threatening
allergic reaction to the penicillin.
